normal human diploid dermal skin fibroblasts  (ATCC)

Journal: Stem cells (Dayton, Ohio)

Article Title: Multipotent Stromal Cells (MSCs) are Activated to Reduce Apoptosis in Part by Upregulation and Secretion of Stanniocalcin-1 (STC-1)

doi: 10.1002/stem.20080742

Figure Lengend Snippet: Table 1

Article Snippet: For the co-culture experiments with irradiated fibroblasts, normal human diploid dermal skin fibroblasts (HS-68; American Tissue Type Culture Collection (ATCC), Rockville, MD) were thawed and plated at 10,000 cells/cm 2 on a 4.6 cm 2 transwell inserts (pore size 0.4 μm, #3450; Corning, New York, NY) in 2 mL growth medium (low glucose DMEM (GIBCO/BRL), 10% FBS and 100 units/mL penicillin).

Techniques: Cell Culture, Irradiation

Journal: African Journal of Traditional, Complementary, and Alternative Medicines

Article Title: Protopine Inhibits Heterotypic Cell Adhesion in MDA-MB-231 Cells Through Down-Regulation of Multi-Adhesive Factors

doi:

Figure Lengend Snippet: Effects of Corydalis yanhusuo extracts and pure compounds on cell viability of HL-60, MCF7, HepG2, MDA-MB-231 and Hs68 cells

Article Snippet: Cell lines and culture Human hepatoma cell line HepG2, human promyelocytic leukemia cell line HL-60, human breast carcinoma cell lines MCF7 and MDA-MB-231, HUVE-12 (Human Umbilical Vein Endothelial Cell-12) and human normal diploid fibroblasts cell line Hs68 were all purchased from American Type Culture Collection (ATCC).

Techniques:

Journal:

Article Title: UV induces nucleolar translocation of ING1 through two distinct nucleolar targeting sequences

doi:

Figure Lengend Snippet: Hs68 fibroblasts were fixed and stained for ING1 (B,E and H) and DNA (A,D and G). Endogenous ING1 (B) is found in the nucleus but not in the nucleolus [which is shown in the differential interference contrast (DIC) images (C,F and I)]. However, when these proteins are overexpressed, they localize mainly to the nucleolus and can also be seen in the nucleoplasm (E). Endogenous ING1 proteins translocate to, and localize within, the nucleolus 48 h after UV-induced DNA damage as shown in (H).

Article Snippet: Cells and DNA constructs Primary normal human diploid fibroblasts (HDFs; Hs68, ATCC CRL#1635) were used for all irradiation studies and both SK-N-SH and Hs68 cells were transfected for overexpression studies.

Techniques: Staining

Journal:

Article Title: UV induces nucleolar translocation of ING1 through two distinct nucleolar targeting sequences

doi:

Figure Lengend Snippet: Mutated versions of p33ING1b (A) were generated and transfected into Hs68 fibroblasts. Cells were visualized (B) by staining for DNA (A,D,G and J), ING1 proteins (B,E,H and K) and by DIC (C,F,I and L).

Article Snippet: Cells and DNA constructs Primary normal human diploid fibroblasts (HDFs; Hs68, ATCC CRL#1635) were used for all irradiation studies and both SK-N-SH and Hs68 cells were transfected for overexpression studies.

Techniques: Generated, Transfection, Staining

Journal:

Article Title: UV induces nucleolar translocation of ING1 through two distinct nucleolar targeting sequences

doi:

Figure Lengend Snippet: Hs68 fibroblasts were transfected with Tat (A–D) or with Tat and GFP–ING1b (E–H). Cells were stained for DNA (A and E), Tat (B and F) and ING1 (C andG). When overexpressed, Tat localizes to the nucleolus (shown by DIC in D) but does not cause relocation of ING1 to the nucleolus (C).

Article Snippet: Cells and DNA constructs Primary normal human diploid fibroblasts (HDFs; Hs68, ATCC CRL#1635) were used for all irradiation studies and both SK-N-SH and Hs68 cells were transfected for overexpression studies.

Techniques: Transfection, Staining

Journal:

Article Title: UV induces nucleolar translocation of ING1 through two distinct nucleolar targeting sequences

doi:

Figure Lengend Snippet: Hs68 fibroblasts were fixed and stained for ING1 (B,E and H) and DNA (A,D and G). Endogenous ING1 (B) is found in the nucleus but not in the nucleolus [which is shown in the differential interference contrast (DIC) images (C,F and I)]. However, when these proteins are overexpressed, they localize mainly to the nucleolus and can also be seen in the nucleoplasm (E). Endogenous ING1 proteins translocate to, and localize within, the nucleolus 48 h after UV-induced DNA damage as shown in (H).

Article Snippet: Primary normal human diploid fibroblasts (HDFs; Hs68, ATCC CRL#1635) were used for all irradiation studies and both SK-N-SH and Hs68 cells were transfected for overexpression studies.

Techniques: Staining

Journal:

Article Title: UV induces nucleolar translocation of ING1 through two distinct nucleolar targeting sequences

doi:

Figure Lengend Snippet: Mutated versions of p33ING1b (A) were generated and transfected into Hs68 fibroblasts. Cells were visualized (B) by staining for DNA (A,D,G and J), ING1 proteins (B,E,H and K) and by DIC (C,F,I and L).

Article Snippet: Primary normal human diploid fibroblasts (HDFs; Hs68, ATCC CRL#1635) were used for all irradiation studies and both SK-N-SH and Hs68 cells were transfected for overexpression studies.

Techniques: Generated, Transfection, Staining

Journal:

Article Title: UV induces nucleolar translocation of ING1 through two distinct nucleolar targeting sequences

doi:

Figure Lengend Snippet: Hs68 fibroblasts were transfected with Tat (A–D) or with Tat and GFP–ING1b (E–H). Cells were stained for DNA (A and E), Tat (B and F) and ING1 (C andG). When overexpressed, Tat localizes to the nucleolus (shown by DIC in D) but does not cause relocation of ING1 to the nucleolus (C).

Article Snippet: Primary normal human diploid fibroblasts (HDFs; Hs68, ATCC CRL#1635) were used for all irradiation studies and both SK-N-SH and Hs68 cells were transfected for overexpression studies.

Techniques: Transfection, Staining